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. 2018 Dec 5;9(12):1165. doi: 10.1038/s41419-018-1149-x

Fig. 7. Identification of NR2C2-binding sites on SPOCK1 and UCA1 promoters.

Fig. 7

a Western blot analysis for SPOCK1 in U87 and U251 cells with the expression of NR2C2 changed. Error bars represent as the mean ± SD (n = 3, each group). **P < 0.01, #P < 0.05. b NR2C2 bound to the promoter of SPOCK1 in U87 and U251 cell lines. Schematic representation of the human SPOCK1 promoter region 3000 bp upstream of the transcription start site (transcription start site (TSS), designated as +1). Polymerase chain reaction (PCR) was conducted with the resulting precipitated DNA. c Relative expression of UCA1 in U87 and U251 cells with the expression of NR2C2 changed. Error bars represent as the mean ± SD (n = 3, each group). **P < 0.01, #P < 0.05. d NR2C2 bound to the promoter of UCA1 in U87 and U251 cell lines. Schematic representation of the human UCA1 promoter region 3000 bp upstream of the transcription start site (TSS, designated as +1). Polymerase chain reaction (PCR) was conducted with the resulting precipitated DNA