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. 2018 Dec 5;8:17676. doi: 10.1038/s41598-018-36149-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transfection efficiency of GPX1-directed siRNA in porcine splenic lymphocytes. the blank control shown in (A), the transfection efficiency of combination was 51.1% + 0.8% shown in (B), the transfection efficiency of combination was 71.3% + 1.3% shown in (C), the transfection efficiency of combination was 80.6% + 1.7% shown in (D), and the transfection efficiency of combination was 92.9% + 2% shown in (E). Therefore, we selected combination E for the subsequent experiment.