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. 2018 Dec 5;8:17676. doi: 10.1038/s41598-018-36149-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative expression of GPX1 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) proteins. Its show that there was no significant difference in the expression of GAPDH between the normal and knockdown groups. However, the expression of GPX1 differed significantly between the normal control and knockdown groups, and that GPX1-knockdown cells expressed only 36.9% of the GPX1 expressed by the normal group. Therefore, the knockdown efficiency was 63.1%. ^##P < 0.01. Table 1. the expression of GPX1 in the group of cells treated with the control GPX1-directed siRNA was 28.4% of that in the control group, whereas the expression of GPX1 in the scrambled siRNA group was 99.4% of that in the control group. This suggests that there was no nonspecific gene silencing.