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. 2018 Dec 5;7:205. doi: 10.1038/s41426-018-0201-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Cytopathic effects at 1dpi displayed on RD cells infected with CMU4232 or CDV-Isehara. b Detection of viral structural proteins VP0 and VP2 of CMU4232 and CDV-Isehara by western blotting with antibody specific for EV-A71. β-actin was used as a internal control. c Growth curves of CMU4232 and CDV-Isehara in RD cells. RD cells were inoculated with CMU4232 or CDV-Isehara as indicated at either MOI of 1 or 0.1. Samples were collected at the times indicated and titrated by PFU assay. All assays were performed in triplicate. At each time point, titer values are means of three samples; error bars represent SEM. dpi days post infection, PFU plaque forming unit, SEM standard error of mean