Table 3.

Association of NLRP3, AIM2, and ASC genes promotor methylation with T2DM.

Gene	CpGs	Methylation levela	T2DM patients vs. healthy controls
			OR (95% CI)b	P-valueb
NLRP3	Total methylation	≧0.764	Ref.
		<0.764	1.08 (0.52~2.24)	0.804
		Per SD less methylation	1.14 (0.78~1.65)	0.499
	CpG1	≧0.823	Ref.
		<0.823	0.47 (0.22~1.02)	0.056
		Per SD less methylation	0.98 (0.68~1.43)	0.924
	CpG2	≧0.778	Ref.
		<0.778	1.77 (0.84~3.73)	0.132
		Per SD less methylation	1.14 (0.78~1.66)	0.498
	CpG3	≧0.851	Ref.
		<0.851	0.99 (0.48~2.07)	0.988
		Per SD less methylation	0.97 (0.66~1.41)	0.860
	CpG4	≧0.607	Ref.
		<0.607	1.44 (0.69~3.03)	0.332
		Per SD less methylation	1.37 (0.92~2.04)	0.118
AIM2	Total methylation	≧0.968	Ref.
		<0.968	2.29 (1.28~6.66)	0.011*
		Per SD less methylation	1.40 (0.94~2.07)	0.096
	CpG2	≧0.911	Ref.
		<0.911	0.91 (0.42~1.97)	0.808
		Per SD less methylation	1.16 (0.80~1.68)	0.446
	CpG3	=1.000	Ref.
		<1.000	8.29 (3.37~20.40)	<0.001**
		Per SD less methylation	1.72 (1.06~2.80)	0.029*
ASC	Total methylation	≧0.929	Ref.
		<0.929	1.21 (0.56~2.61)	0.621
		Per SD less methylation	1.10 (0.75~1.59)	0.641
	CpG1	≧0.912	Ref.
		<0.912	1.35 (0.62~2.93)	0.453
		Per SD less methylation	1.17 (0.80~1.69)	0.426
	CpG2	≧0.951	Ref.
		<0.951	1.14 (0.53~2.43)	0.741
		Per SD less methylation	0.96 (0.66~1.41)	0.844
	CpG3	≧0.922	Ref.
		<0.922	1.30 (0.61~2.78)	0.504
		Per SD less methylation	1.13 (0.78~1.64)	0.511

^aMedian of total or each CpG methylation in healthy controls.

^bOR, P-values were from logistic regression analyses controlling for BMI.

^*P < 0.05,

^**P < 0.001; The bold in the table means statistically significant. (P < 0.05).

Per SD less methylation, the decrease of per standard deviation of CpG methylation, the change of OR value.