. 2018 Nov 29;9:709. doi: 10.3389/fendo.2018.00709

Table 4.

Association of NLRP3, AIM2, and ASC genes promotor methylation with T2DM vascular complications.

Gene	CpGs	Methylation level^a	T2DM with microvascular complications vs. T2DM without any complication		T2DM with macrovascular complications vs. T2DM without any complication		T2DM with both micro-macrovascular complications vs. T2DM without any complication
			OR (95% CI)^b	P-value^b	OR (95% CI)^b	P-value^b	OR (95% CI)^b	P-value^b
NLRP3	Total methylation	≧0.782	Ref.		Ref.		Ref.
		< 0.782	1.11 (0.26~4.78)	0.890	36.03 (3.11~417.06)	0.004^*	17.78 (2.04~155.28)	0.009^*
		Per SD less methylation	1.21 (0.56~2.63)	0.623	2.00 (0.88~4.55)	0.098	2.19 (1.01~4.76)	0.048^*
	CpG1	≧0.859	Ref.		Ref.		Ref.
		< 0.859	1.06 (0.25~4.57)	0.936	3.83 (0.82~17.87)	0.088	4.34 (0.83~22.73)	0.083
		Per SD less methylation	1.20 (0.50~2.87)	0.684	1.17 (0.69~1.99)	0.568	1.39 (0.71~2.72)	0.338
	CpG2	≧0.790	Ref.		Ref.		Ref.
		< 0.790	6.45 (1.05~39.78)	0.045^*	14.90 (1.98~111.96)	0.009^*	17.78 (2.06~155.28)	0.009^*
		Per SD less methylation	0.60 (0.24~1.49)	0.266	3.98 (1.23~12.95)	0.022^*	3.75 (1.38~10.18)	0.010^*
	CpG3	≧0.865	Ref.		Ref.		Ref.
		< 0.865	0.69 (0.15~3.26)	0.641	6.09 (1.13~32.78)	0.036^*	3.46 (0.71~16.93)	0.125
		Per SD less methylation	1.18 (0.51~2.77)	0.696	4.07 (1.37~12.05)	0.011^*	3.22 (1.27~8.16)	0.014^*
	CpG4	≧0.600	Ref.		Ref.		Ref.
		< 0.600	0.27 (0.05~1.43)	0.123	2.69 (0.64~11.38)	0.178	2.73 (0.59~12.74)	0.202
		Per SD less methylation	1.65 (0.82~3.30)	0.160	1.47 (0.69~3.14)	0.317	1.65 (0.72~3.80)	0.238
AIM2	Total methylation	≧0.971	Ref.		Ref.		Ref.
		< 0.971	9.48 (1.14~79.00)	0.038^*	30.90 (2.59~368.49)	0.007^*	306.22 (2.29~1729.76)	0.006^*
		Per SD less methylation	0.58 (0.27~1.24)	0.157	3.16 (1.25~7.97)	0.015^*	9.55 (1.97~46.24)	0.005^*
	CpG2	≧0.919	Ref.		Ref.		Ref.
		< 0.919	2.51 (0.45~14.07)	0.294	7.46 (1.28~43.37)	0.025^*	5.91 (1.07~32.71)	0.042^*
		Per SD less methylation	0.65 (0.32~1.29)	0.215	2.91 (1.13~7.55)	0.028^*	3.40 (1.05~10.99)	0.041^*
	CpG3	≧1.000	Ref.		Ref.		Ref.
		< 1.000	2.66 (0.05~12.48)	0.226	4.41 (0.94~20.61)	0.059	257.63 (5.34~1429.38)	0.005^*
		Per SD less methylation	0.67 (0.30~1.48)	0.321	1.94 (0.93~4.08)	0.080	8.27 (2.24~30.55)	0.002^*
ASC	Total methylation	≧0.939	Ref.		Ref.		Ref.
		< 0.939	1.00 (0.23~4.26)	0.996	NA	NA	2.60 (0.49~13.83)	0.262
		Per SD less methylation	0.80 (0.28~2.30)	0.686	35.78 (3.67~349.19)	0.002^*	4.43 (1.19~16.45)	0.026^*
	CpG1	≧0.931	Ref.		Ref.		Ref.
		< 0.931	2.62 (0.55~12.40)	0.225	NA	NA	12.69 (1.24~130.05)	0.032^*
		Per SD less methylation	1.04 (0.37~2.88)	0.943	69.22 (4.05~1620.25)	0.003^*	10.86 (1.57~75.11)	0.016^*
	CpG2	≧0.975	Ref.		Ref.		Ref.
		< 0.975	2.74 (0.57~13.14)	0.209	38.91(2.26~1183.45)	0.012^*	2.33 (0.47~11.64)	0.302
		Per SD less methylation	0.53 (0.16~1.72)	0.290	8.62 (2.31~32.15)	0.011^*	2.45 (0.84~7.13)	0.101
	CpG3	≧0.949	Ref.		Ref.		Ref.
		< 0.949	1.72 (0.39~7.62)	0.474	NA	NA	11.28 (1.13~112.33)	0.039^*
		Per SD less methylation	1.01 (0.46~2.24)	0.980	5.74 (1.69~19.47)	0.005^*	1.92 (0.85~4.35)	0.119

Median of total or each CpG methylation in T2DM without any complications.

OR, P-values were from logistic regression analyses controlling for age.

P < 0.05; The bold in the table means statistically significant. (P < 0.05).

NA, not available because of zero cases in case group, grouped by methylation median of control group; Per SD less methylation, the decrease of per standard deviation of CpG methylation, the change of OR value.