Table 5.
Orthogonal partial least squares regression analysis model of CON and Age.
| Spot number | Protein Name | Accession number | Biological process | Experimental MW (kDa)/pI | VIP | p(corr) | OD CON (Mean ± SD) | OD CWP (Mean ± SD) | OD quotient mean | Alteration CWP vs. CON |
|---|---|---|---|---|---|---|---|---|---|---|
| 6505 | Ig alpha-2 chain C region | P01877 | Immunity | 94 / 6.02 | 1.53 | −0.62 | 2614 ± 1651 | 1772 ± 1267 | 0.68 | ↓ |
| 3406 | Antithrombin-III | P01008 | Metabolic | 76 / 5.54 | 1.52 | 0.65 | 1243 ± 671 | 973 ± 734 | 0.78 | ↓ |
| 8618 | Fibrinogen alpha chain | P02671 | Metabolic | 105 / 7.47 | 1.51 | −0.65 | 2045 ± 808 | 2365 ± 1147 | 1.16 | ↑ |
| 5319 | Haptoglobin | P00738 | Inflammatory | 54 / 5.88 | 1.28 | 0.60 | 388 ± 232 | 456 ± 539 | 1.18 | ↑ |
| 8719 | Fibrinogen alpha chain | P02671 | Metabolic | 107 / 7.11 | 1.28 | −0.53 | 3726 ± 1058 | 4040 ± 1837 | 1.08 | ↑ |
| 6407 | Beta-2-glycoprotein 1 | P02749 | Lipid metabolism | 81 / 6.00 | 1.27 | 0.60 | 567 ± 125 | 651 ± 299 | 1.15 | ↑ |
| 7120 | Ig kappa chain C region | P01834 | Immunity | 28 / 6.29 | 1.23 | −0.47 | 7997 ± 4977 | 6563 ± 6880 | 0.82 | ↓ |
| 4316 | Haptoglobin | P00738 | Inflammatory | 61 / 5.47 | 1.20 | 0.54 | 12243 ± 5754 | 12341 ± 7702 | 1.01 | ↑ |
| 2601 | Alpha-1B -glycoprotein | P04217 | Metabolic | 107 / 5.36 | 1.19 | 0.54 | 3245 ± 838 | 3063 ± 434 | 0.94 | ↓ |
| 7743 | Serotransferrin | P02787 | Iron ion homeostasis | 120 / 6.29 | 1.18 | −0.54 | 32454 ± 8480 | 26409 ± 6738 | 0.81 | ↓ |
| 6114 | Ig kappa chain C region | P01834 | Immunity | 28 / 6.05 | 1.12 | −0.41 | 1733 ± 2089 | 640 ± 883 | 0.37 | ↓ |
| 6840 | Complement C3 alpha chain | P01024 | Immunity | 157 / 6.08 | 1.09 | 0.54 | 386 ± 169 | 614 ± 904 | 1.59 | ↑ |
| 5821 | Alpha-2 -macroglobulin | P01023 | Immunity | 176 / 5.71 | 1.08 | −0.49 | 407 ± 228 | 579 ± 284 | 1.42 | ↑ |
| 3105 | Haptoglobin | P00738 | Inflammatory | 54 / 5.47 | 1.05 | 0.45 | 2076 ± 1223 | 2770 ± 1183 | 1.33 | ↑ |
| 2202 | Alpha-1 -antitrypsin | P01009 | Metabolic | 69 / 5.36 | 1.04 | −0.43 | 3372 ± 1579 | 2829 ± 2183 | 0.84 | ↓ |
| 6731 | Serotransferrin | P02787 | Iron ion homeostasis | 130 / 6.25 | 1.03 | −0.32 | 2008 ± 714 | 1798 ± 710 | 0.90 | ↓ |
| 4833 | Ceruloplasmin | P00450 | Iron ion homeostasis | 163 / 5.51 | 1.02 | 0.37 | 81 ± 109 | 219 ± 160 | 2.70 | ↑ |
| 2203 | Haptoglobin | P00738 | Inflammatory | 61 / 5.36 | 1.02 | 0.44 | 12006 ± 6246 | 16032 ± 7681 | 1.34 | ↑ |
| 4301 | Fibrinogen gamma chain | P02679 | Metabolic | 72 / 5.47 | 1.00 | 0.50 | 4565 ± 2265 | 6308 ± 3044 | 1.38 | ↑ |
19 proteins had a VIP > 1. The 5 proteins with highest VIP values (bold marking) is considered as most important regressor for the model and belonged to immunity, inflammatory and metabolic processes. SD, standard deviation; CON, control; CWP, chronic widespread pain; OD, optical density; MW, molecular weight; pI, isoelectric point; VIP, variable influence on projection; OPLS, orthogonal partial least squares regression analysis.