Figure 4.

Early metformin treatment rescued the repetitive behaviors in BTBR mice. (A) BTBR mice showed severe repetitive behaviors due to high levels of self-grooming compared to B6 mice, and early metformin treatment can reverse it. (B) Metformin treatment tended to reduce grooming bouts in BTBR mice compare with saline-treated BTBR mice, the effect approached statistical significance. (C) BTBR mice showed decreased proportion of incorrect transitions compared to B6 mice, and metformin treatment increased it. (D) BTBR mice buried more marbles compared to B6 mice, and metformin treatment can reverse it. Data are presented as the means ± SEM. n = 10. *P < 0.05, **P < 0.01.