Figure 7.

Expression of mTOR, S6K, NF-κB and the protein levels of mTOR in the cerebral cortex of B6 and BTBR mice. (A) BTBR mice showed an increased expression of mTOR in the cerebral cortex, and metformin treatment could decrease mTOR mRNA level in BTBR mice. (B) BTBR mice showed an increased expression of S6K in the cerebral cortex, and a decreased trend of S6K level was seen after metformin treatment. (C) The expression of NF-κB was not affected by the metformin treatment. Data are presented as the means ± SEM. n = 4. **P < 0.01, *P < 0.05. (D) Representative western blot image for mTOR protein expression. (E) Quantitative data of mTOR protein expression in the cortex of the mice. Data are expressed as the means ± SEM (n = 3 in each group; **P < 0.01).