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. Author manuscript; available in PMC: 2019 Dec 4.
Published in final edited form as: Cell Metab. 2018 Aug 30;28(6):833–847.e8. doi: 10.1016/j.cmet.2018.08.008

Figure 3. MtCK1 Y153 phosphorylation promotes TRAP1 dissociation and protection from proteasomal degradation.

Figure 3.

(A) Immunoblots of lysates (left) and qRT-PCR for MtCK1 mRNA (right) from “rescued” BT474 cells that stably express Flag-MtCK1 WT or Y153F with stable knockdown of endogenous MtCK1. (B) Immunoblots of “rescued” BT474 cells expressing Flag-MtCK1 WT and Y153F after treatment with MG132 for 6 h. (C) Immunoblots of anti-Flag immunoprecipitates and whole-cell lysates (WCL) from Flag-MtCK1 WT and Y153F “rescued” BT474 cells. (D) Immunoblots (WB) of anti-TRAP1 or anti-IgG immunoprecipitates and whole-cell lysates (WCL) from BT474 cells with or without lapatinib treatment (500 nM, 6 h). (E) Immunoblots (WB) of anti-Flag immunoprecipitates and WCL from 293T cells expressing Flag-MtCK1 WT expressing control vector (−) or HER2. (F) Immunoblots of lysates (left) and PCr levels (right) from 293T cells expressing vector control (−) or HER2 with or without stable knockdown of endogenous TRAP1. All results are representative experiments of three independent replicates. P values were determined by a two-tailed Student’s t test (*P<0.05).