|
Escherichia coli M15 [pREP4] (K12) (NaIS, StrS, RifS, Thi–, Lac–, Ara+, Gal+, Mtl–, F–, RecA+, Uvr+, Lon+) |
Qiagen |
Cat. #34210 |
| Chemicals, Tissues, Peptides, and Recombinant Proteins |
| Luria-Bertani powder |
Fisher |
Cat. #BP9723 |
| Bacto Agar |
DIFCO |
Cat. #214050 |
| Kanamycin |
Sigma |
Cat. #K4000 |
| Carbenicillin disodium salt |
Alfa Aesar |
Cat. #J61949 |
| Magnesium sulfate hexahydrate |
Baker |
Cat. #1–2500 |
| Potassium chloride |
Sigma |
Cat. #P4504 |
| Isopropyl-β-D-thiogalactopyranoside (IPTG) |
RPI Corp. |
Cat. #I-56000 |
| Nickel-nitrilotriacetic acid (NiNTA) agarose |
Qiagen |
Cat. #30210 |
|
Tris(hydroxymethyl)aminomethane |
Sigma |
Cat. #T1503 |
| Sodium chloride |
Sigma |
Cat. #S9625 |
| Imidazole |
Sigma |
Cat. #I2399 |
| Bacterial Protein Extraction Reagent (B-per) |
Thermo |
Cat. #78248 |
| HALT protease inhibitor |
Thermo |
Cat. #78439 |
| Pepstatin A |
Santa Cruz Biotech |
Cat. #SC-45036A |
| Dimethylsulfoxide (DMSO) |
Sigma |
Cat. #D2650 |
| Phenylmethylsulfonyl fluoride (PMSF) |
Sigma |
Cat. #P7626 |
| E-64 protease inhibitor |
Fisher |
Cat. #501010687 |
| 2-mercaptoethanol |
Bio-Rad |
Cat. #161–0710 |
| cOmplete Mini protease inhibitor tablets – EDTA free |
Sigma |
Cat. #11836170001 |
| Lysozyme |
Sigma |
Cat. #L6876 |
| DNase I recombinant |
Sigma |
Cat. #04536282001 |
| Triton X-100 |
Fisher |
Cat. #BP151 |
| Sodium phosphate dibasic |
Fisher |
Cat. #S374 |
| Pig hearts |
Pel Freez |
Cat. #59416–1 |
| Recombinant DNA |
| pQE-2 vector |
Qiagen |
Cat. #32932 |
| Zero-Blunt vector kit |
Invitrogen |
Cat. #K2750 |
| pQE-2 vector |
Qiagen |
Cat. #32932 |
| Zero-Blunt vector kit |
Invitrogen |
Cat. #K2750 |
| Deposited Data |
| NMR structure of domain cC0 of cardiac myosin binding protein C |
(Ratti et al., 2011) |
PDB ID 2K1M |
| Crystal structure of the C1 domain of cardiac myosin binding protein-C |
Govada et al., 2008) |
PDB ID 2V6H |
| Pseudo-atomic model of the myosin-Tpm-F-actin complex |
(Behrmann et al., 2012) |
PDB ID 4A7F |
| Pseudo-atomic model of the myosin-Tpm-F-actin complex |
(von der Ecken et al., 2016) |
PDB ID 5JLH |
| Pseudo-atomic model of the cardiac open (c-open) structural state of the cardiac TF |
(Risi et al., 2017) |
PDB ID 5NOJ |
| C1 bound to the TF in C1-S mode |
This paper |
EMD-4346, PDB ID 6G2T |
| C0C1 bound to the TF in the C0-F1+C1-S mode |
This paper |
EMD-7780, PDB ID 6CXI |
| C0C1 bound to the TF in the C0-F2+C1-S mode |
This paper |
EMD-7781, PDB ID 6CXJ |
| Software and Algorithms |
| SPIDER |
(Frank et al., 1981) |
https://spider.wadsworth.org |
| CTFFIND3 |
(Mindell et al., 2003) |
http://grigoriefflab.janelia.org |
| EMAN |
(Ludtke et al., 1999) |
http://blake.bcm.edu/emanwiki |
| IHRSR |
(Egelman, 2000) |
egelman@virginia.edu |
| DireX |
(Schroder et al., 2007) |
https://simtk.org/home/direx |
| CNS |
(Adams et al., 2010) |
http://cns-online.org |
| UCSF Chimera |
(Pettersen et al., 2004) |
http://www.cgl.ucsf.edu/chimera |
| ExPASy |
SIB Swiss Institute of Bioinformatics |
https://web.expasy.org/protparam |
