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Manipulating expression of silent biosynthetic genes using CRISPR-Cas9. (a) CRISPR-Cas9 methods adapted for use in streptomycetes are used to insert an active uni- or bi-directional promoter to drive expression of silent BGCs. (b) This approach has been used in S. roseosporus and S. viridochromogenes, among other strains, to induce production of alteramide A, FR-9000098, and a type II PKS-derived pigment.
