Figure 3.
High-throughput elicitor screening in S. albus. The cryptic sur cluster was targeted with two reporter strains, one where the promoter-reporter construct Psur-eGFPx3 was inserted into a neutral chromosomal site and a second, in which eGFPx3 was incorporated site-specifically into the sur cluster downstream of Psur. HiTES with these reporters revealed etoposide and ivermectin as inducers of the sur cluster, which was subsequently shown to produce a bouquet of metabolites, including surugamides, acyl-surugamide A, albucyclones, and surugamide F variants. Both elicitors globally enhanced secondary metabolite synthesis, leading to the identification of a new metabolite, albuquinone A.