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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Curr Opin Microbiol. 2018 Jun 5;45:156–163. doi: 10.1016/j.mib.2018.05.001

Figure 4.

Figure 4

Reporter-guided mutant selection in Streptomyces spp. A double reporter construct, xylE-neo, is placed under the control of a desired silent promoter and the strain is then mutagenized using UV, chemical or transposon mutagenesis. Desired mutants are selected on Kan plates and further by screening for catecholase-positive colonies. The metabolome of the mutant and wt strain are then compared by HPLC-MS to identify the products of the chosen silent cluster. This approach led to overproduction of jadomycin B and gaudimycin D, among other products.