Skip to main content
. Author manuscript; available in PMC: 2019 Aug 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2018 Jun 6;353:23–30. doi: 10.1016/j.taap.2018.06.003

Fig. 1. Exposure of BEAS-2BR cells to cadmium generated ROS and antioxidants decreased the generation.

Fig. 1

(A) ROS levels from BEAS-2BR cells exposed to cadmium at indicated concentrations of cadmium were measured using flow cytometry. (B) ESR spectra were recorded from BEAS-2BR cells exposed to cadmium at different experimental conditions. The concentrations of the reagents used are: 0 and 10 µM CdCl2, 10 µM CdCl2 with 10 µM sulforaphane (SFN). Spectrometer settings were: receiver gain, 1.25×105; modulation amplitude, 1.25 G; scan time 200 s; field 3480 + 50 G; and time constant, 1.5 seconds. 5,5-dimethyl-1-pyrroline N-oxide (DMPO) was used as a spin (radical) trapping agent.