Fig. 5. Inhibition of cadmium-induced cell transformation by overexpression of antioxidant enzymes.
BEAS-2BR cells or BEAS-2BR cells with overexpression of catalase (CAT), superoxide dismutase 1 (SOD1), or superoxide dismutase (SOD2) were exposed to 2 µM CdCl2. Fresh medium was added every 3 days. Cell transformation (soft agar) assay was performed as described in in our previous studies (Son et al., 2012). The anchorage-independent formation of colonies was stained with 1 mg/mL iodonitrotetrazolium violet overnight.