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Preparation of control B. glabrata hemocyte aggregates and hemocyte-encapsulated S. mansoni sporocysts for cryohistology. Hemocyte-sporocyst encapsulations (arrows) following larval-hemolymph coculture for 18 h and washing in CBSS (A); Isolated hemocyte samples placed in a cryo-mold (insert hemocyte-sporocyst aggregate) (B); Quick freezing of cellular encapsulations in OTC prior to cryosectioning (C).
