Table 1.
Primers used in this experiment.
| primer name | sequence (5′ to 3′) | purpose |
|---|---|---|
| Δ9FAD_qPCR_F | AACCCCTACCTGGGCTTCATCT | quantitative RT-PCR |
| Δ9FAD_qPCR_R | ATGCGGGTGTAGGCAATCTCG | |
| Δ12FAD_qPCR_F | ATGCGGGTGTAGGCAATCTCG | |
| Δ12FAD_qPCR_R | GGGAGGAGACGCTGAAGAAGAG | |
| Δ15FAD_qPCR_F | CGAGGGCTCCCACTTCGACC | |
| Δ15FAD_qPCR_R | TCCAGCCACATCACAAACACCC | |
| β-Actin_F | GCTCAACTCCTCCACGCT | |
| β-Actin_R | GTCCTTGCGGATGTCCAC | |
| Δ9FAD_F | GACAAGTTTGCAGAGGAGCAG | gene cloning |
| Δ9FAD_R | TCAGACGGACACCTCGCGGT | |
| Δ9FAD_BamHI_F | CGCGGATCCATGGACAAGTTTGCAGAGGAGCAG | construction of expression vector for E. coli |
| Δ9FAD_HindIII_R | CCCAAGCTTTCAGACGGACACCTCGCGGT | |
| Δ9FAD_HindIII_F | CCCAAGCTTATGGACAAGTTTGCAGAGGAGCAG | construction of expression vector for S. cerevisiae |
| Δ9FAD_AscI_R | TATTGGCGCGCCTCAGACGGACACCTCGCGGT |