Figure 3.

Photographs of B. subtilis (a) and P. aeruginosa (b) colonies cultivated on agar culture plates with different concentrations of rGO (0.01–0.08%) with seeded bacterial concentrations of 10⁶ CFU ml⁻¹ (a(i),b(i)); measured bacterial biofilm area of each agar culture plate (a(ii),b(ii)). Sigmoidal plots of B. subtilis and P. aeruginosa with rGO. Inhibition of B. subtilis colony biofilm formation with increasing concentrations of rGO followed first order of exponential decay (a(iii),b(iii)).