Figure 4.

Frequency changes of different regulatory T cell populations. (A) Regulatory T cells defined as CD4⁺ CD25⁺ CD127⁻ or (B) FoxP3⁺ CD4⁺ CD25⁺ CD127⁻. (C) t-Distributed Stochastic Neighbor Embedding (t-SNE) plot for the expression-distribution of CD25, CD127, FoxP3, CD45RA, CD31 and CD39 within one representative sample (red ellipses indicate regulatory T cells). (D) CD39⁺ CD31⁻ and (E) CD39⁻ CD31⁺ Tregs defined as being CD25⁺ CD127⁻ CD4⁺. (F) Memory phenotype CD31⁺ FoxP3⁺ CD45RA⁻ Tregs and (G) naïve phenotype CD39⁺ FoxP3⁺ CD45RA⁺ Tregs in MS patients after 4 weeks of normoxic (NO, n = 16, black circles) and hypoxic (HO, n = 14, gray circles) treadmill training. Three samples not analyzed due to quality issues. P-values by Student's paired t-test or Wilcoxon signed rank test.