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. 2018 Nov 29;12:872. doi: 10.3389/fnins.2018.00872

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Copyright © 2018 Hanson, Tian, Vickers and King.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Axon degeneration and loss in microfluidic chambers after kainic acid treatment. Live imaging of the axonal compartment of microfluidic chambers were analyzed for (A) axon loss and (B) axon degeneration index after treatment with 10 or 25 μM kainic acid. Axon loss was significantly increased (p < 0.05) following 25 μM kainic acid treatment relative to control, whereas, 10 μM kainic acid did not cause significant axon loss compared to control. The axon degeneration index was significantly increased (p < 0.05) following either 10 or 25 μM doses of kainic acid compared to control. Bar graph represents mean ± SEM *p < 0.05 relative to control.