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. 2018 Nov 29;12:872. doi: 10.3389/fnins.2018.00872

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Copyright © 2018 Hanson, Tian, Vickers and King.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes to microtubules after kainic acid treatment. Changes to microtubule post-translational modifications and the microtubule-associated protein tau were investigated in both whole cultured neurons and isolated axons after treatment with 25 μM kainic acid. ELISA analysis of neurons at 1 and 6 h after 25 μM kainic acid treatment showed (A) a significant decrease (p < 0.05) in acetylated tubulin levels after 6 h and (B) a significant decrease (p < 0.05) in tau levels at both 1 and 6 h, relative to control. (C) Tyrosinated tubulin levels were unchanged compared to control. ELISA analysis of axons at 6 h post treatment with 25 μM kainic acid demonstrated (D) significantly decreased (p < 0.05) levels of acetylated tubulin, whereas (E) tau axonal levels were significantly increased (p < 0.05) at this timepoint relative to control. Axonal levels of (F) tyrosinated tubulin were unchanged compared to control. To confirm the effect of 25 μM kainic acid on acetylated tubulin in axons Western blot analysis was performed (G) demonstrating a significant decrease (p < 0.05). Bar graph represents mean ± SEM *p < 0.05 relative to control.