Neisseria meningitidis serogroup B (NmB) carriage prevalence rates at each visit for both cohorts. NmB carriage was determined by seroagglutination, isolate PCR, and direct swab PCR assays at 3 visits and any visit for ninth-grade students (A) and university students (C) and by isolate whole-genome sequencing (WGS), isolate PCR, direct swab PCR, and live cell phenotypic assay (LCPA) for eleventh-grade/college entry students (B). *, P < 0.05. CIs were calculated using the exact method based on Clopper-Pearson (2-sided). The McNemar test using the exact method was used to compare the prevalence rates between PCR analyses and seroagglutination for each visit in ninth-grade/university students.