Skip to main content
. 2018 Nov 29;9:2823. doi: 10.3389/fimmu.2018.02823

Figure 2.

Figure 2

(A) CHAC1 mRNA expression in NCI-H292 cells stimulated with 1 μg/mL LPS and/or 50 ng/mL flagellin for 6 h. Data are expressed as the means ± SEMs of five independent experiments. Statistical analysis was performed using analysis of variance followed by Dunnett's multiple comparison test (control group: NS, *p < 0.05; ***p < 0.001; ****p < 0.0001). (B) CHAC1 mRNA expression in NCI-H292 cells infected with PAK WT, PAK ΔFliC, or PAK ΔpscFΔxcpQ (MOI 0.25) for 6 h. Data are expressed as means ± SEMs of four independent experiments. Statistical analysis was carried out using analysis of variance followed by Bonferroni's multiple comparison test (control group: NS, *p < 0.05; ****p < 0.0001). (C) CHAC1 mRNA expression in NCI-H292 cells infected with PAK strain (MOI 0.25) or different clinical strains of Pa (MOI 0.25) for 6 h. Data are expressed as means ± SEMs of four independent experiments (NS, diamonds; PAK, squares; clinical strains, triangles). Statistical analysis was carried out using analysis of variance followed by Bonferroni's multiple comparison test (control group: NS, **p < 0.01). (D) CHAC1 (black) and IL-8 (gray) mRNA expression in NCI-H292 cells infected with PAK, Staphylococcus aureus (S. aureus), or Stenotrophomonas maltophilia (S. maltophilia, MOI 0.25) for 6 h. Data are expressed as means ± SEMs of three independent experiments. Statistical analysis was carried out using analysis of variance followed by Dunnett's multiple comparison test (control group: NS, ##p < 0.01 [IL-8]; ****p < 0.0001 [CHAC1]). (E) CHAC1 and β-actin protein expression and CHAC1-GFP fluorescence in NCI-H292 cells transfected with increasing amounts of CHAC1-GFP plasmid. (F) CHAC1 and β-actin protein expression in NCI-H292 cells treated with increasing concentrations of MG-132 for 6 h.