. 2018 Oct 16;5(12):555–565. doi: 10.15698/mic2018.12.661

Table 2.

Summary of phenotypic characteristics of the engineered sepJ mutants.

Phenotypic group	Mutant strains		Filamentation^a		Diazotrophic performance^b	Calcein transfer^c
Phenotypic group	Name	SepJ protein	BG11	BG11₀	Diazotrophic performance^b	Calcein transfer^c
Parental strain	CSVM90	Δ_463-748	-	-	-	(+)
1	CSFR21	A₅₄₂R	-	-	-	(++)
1	CSFR22	Δ_739-751	-	-	-	(++)
2	CSFR16	H₆₂₄A	[+]	-	[+]	(+)
2	CSFR26	T₆₁₆A	+	-	+	(+)
3	CSFR12	Δ_498-507	+	+	+	(+)
	CSFR13	R₆₁₇A	+	+	+	(+)
	CSFR25	Y₆₁₂A	+	+	+	(+)
	CSFR27	R₅₆₂A	+	+	+	(+)
4	CSFR14	E₆₆₃A	+	+	+	+++
	CSFR15	S₆₆₇A	+	+	+	+++
	CSFR19	G₅₇₉A	+	+	+	+++
	CSFR20	G₇₂₄A	+	+	+	+++
	CSFR28	E₅₈₀A	+	+	+	+++
Reconstituted wild type	CSFR11	WT SepJ	+	+	+	+++

Data from Fig. 3: +, normal filament length; [+], intermediate filament length; -, extensive filament fragmentation.

Diazotrophic performance summarizes diazotrophic growth (Fox phenotype, from Fig. 5), nitrogenase activity, and production of heterocysts (from Table 1). +, positive; [+], weak positive; -, negative.

Calcein transfer is summarized as: +++, normal (not significantly different from the CSFR11 control); (++), defective (61 to 69 % of the CSFR11 control; note that the difference between strains CSFR21 and CSFR11 could not be defined as statistically significant); (+), defective (37 to 47 % of the CSFR11 control; 32 % in the case of CSVM90) (see text and Table 1 for details).