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. Author manuscript; available in PMC: 2019 Oct 4.
Published in final edited form as: Cell Stem Cell. 2018 Sep 20;23(4):516–529.e5. doi: 10.1016/j.stem.2018.08.009

Figure 6. Conserved roles for BMP and NOTCH signaling in the differentiation of hPSC-derived EPCs.

Figure 6.

(A-B) BMP4 treatment promotes the squamous differentiation of RUES2-derived EPCs. Data represent mean ± SEM (n = 4). *p < 0.05 by unpaired, two-tailed Student’s t test. (C) Enrichment of Jag1, Jag2 and receptors in the epithelium of E12.5 mouse esophagus (n=3) and skin, human fetal esophageal epithelia (n=4) and RUES2-derived EPCs (n=3). FPKM, Fragments Per Kilobase of transcript per Million mapped reads. (D) DAPT treatment leads to the reduced expression of KRT13 and INVOLUCRIN in EPCs. Data represent mean ± SEM (n = 3). *p < 0.05 by unpaired, two-tailed Student’s t test. (E-G) Deletion of RBPjκblocks the formation of the stratified squamous epithelium in the esophagus of Shh-Cre; RBPjκloxp/loxp mutants (n = 4). Note the reduced thickness of the suprabasal layers (KRT13+ KRT4+) in mutants. Scale bars: 20 μm. See also Figure S6.