FIGURE 1.

Tolerance analysis of yeast expressing ChACBP1 to various abiotic stresses. Yeast cells containing either pYES2 empty vector or pYES2-ChACBP1 were incubated as described in Materials and Methods. Serial dilutions were spotted onto yeast extract/peptone/galactose solid plates supplemented with NaHCO₃, NaCl, H₂O₂, or CuCl₂ at indicated concentrations. Solid yeast extract/peptone/glucose media (YPD) was the control. Growth was monitored for 3–7 days at 30°C. In the cold stress treatment, yeast cells were grown for 15 days at 10°C.