Figure 1.

Mitochondrial morphology and mtDNA of fixed fibroblasts and live fibroblasts co-cultured with MSCs. (A) Representative images demonstrating assessment of mitochondrial morphology and mtDNA of fixed fibroblasts from controls and patients with clinically diagnosed mitochondrial disease. Images were collected using immunofluorescence against TOMM20 (mitochondria) and DNA (DNA). (B) Representative images demonstrating assessment of mitochondrial morphology and mtDNA of live fibroblasts (from controls and patients with a clinically diagnosed mitochondrial disease) co-cultured with MSCs. Images were collected following fluorescent labeling of fibroblasts with MitoTracker Deep Red (mitochondria) and PicoGreen (DNA). MSCs were separately fluorescently labeled with MitoTracker Red (mitochondria) prior to co-culture. Images from both panels were collected using confocal microscopy.