Figure 4.

MSC detection using sequence-specific qualitative PCR. Liver tissue homogenates were used to detect mouse and human genomic DNA 24 h following control (saline) or MSC therapy into C57BL/6 mice. (A) Mouse—forward and common reverse PCR primers for prostaglandin E receptor 2 (PTGER2) were amplified to detect the presence of mouse-specific genetic material. (B) Human—forward and common reverse PCR primers for a non-homologous region of PTGER2 were amplified to detect the presence of human-specific genetic material. Human control samples were isolated from a cultured human cell line. NTC, no template control.