Table 3.
Verification of microarray using qRT-PCR on genes randomly selected from both upregulated/downregulated categories.
| Systematic name | Gene symbol | Gene name | Fold change | |
|---|---|---|---|---|
| Microarray | qRT-PCR | |||
| UPREGULATED | ||||
| NM_009117 | SAA1 | Serum amyloid A 1 | 6.549 | |
| NM_008491 | LCN2 | Lipocalin 2 | 3.333 | 4.154 |
| NM_008768 | ORM1 | Orosomucoid 1 | 3.071 | |
| NM_009690 | CD5l | CD5 antigen-like | 1.747 | |
| NM_010130 | ADGRE1 | Adhesion G protein-coupled receptor E1 | 1.624 | |
| NM_010745 | LY86 | Lymphocyte antigen 86 | 1.621 | |
| NM_011315 | SAA3 | Serum amyloid A 3 | 1.615 | 1.567 |
| NM_008509 | LPL | Lipoprotein lipase | 1.582 | 1.989 |
| NM_008533 | CD180 | CD180 antigen | 1.576 | |
| DOWNREGULATED | ||||
| NM_206537 | CYP2C54 | Cytochrome P450, family 2, subfamily c, polypeptide 54 | 2.739 | |
| NM_010011 | CYP4A10 | Cytochrome P450, family 4, subfamily a, polypeptide 10 | 2.259 | |
| NM_007822 | CYP4A14 | Cytochrome P450, family 4, subfamily a, polypeptide 14 | 2.044 | |
| NM_009993 | CYP1A2 | Cytochrome P450, family 1, subfamily a, polypeptide 2 | 1.713 | |
| NM_010004 | CYP2C40 | Cytochrome P450, family 2, subfamily c, polypeptide 40 | 1.663 | |
| NM_012006 | ACOT1 | Acyl-CoA thioesterase 1 | 1.567 | 2.132 |
| NM_026159 | RETSAT | Retinol saturase (all trans retinol 13,14 reductase) | 1.395 | 1.116 |
| NM_011169 | PRLR | Prolactin receptor | 1.362 | 1.919 |
| NM_019975 | HACl1 | 2-Hydroxyacyl-CoA lyase 1 | 1.347 | |
All qRT-PCR data are presented as mean fold change with β-actin as a loading control. The corresponding fold change from microarray experiments is shown for reference. All data represent HFM vs. HFS (n = 7 for both groups).