Figure 6.

Overall architecture of the structures around and inside the flagellar pocket of Δift140 cells. (a) Three-dimensional model from a serial tomogram, showing the position of the FAZ filament, microtubule quartet (MtQ) and collar filaments relative to the flagellar axoneme (Ax). (b,c) Two cytoplasmic microtubules that emerge from the proximal end of the FAZ filament, as seen in tomogram sections (b,d) and in three-dimensional models (c). While one cytoplasmic microtubule is short (arrow in d), the other runs towards the posterior past the Golgi, and beyond the boundary of the tomogram. (e) Accumulation of membrane-bound structures (dark blue) in the flagellar pocket lumen. These structures did not appear connected to the flagellar membrane (cyan). (f) Membrane-bound structure linked to the flagellar pocket membrane via clear attachment plaques. (g) Thin-section TEM image showing membrane being released from the tip of the flagellum. Scale bars, 200 nm (a–e); 50 nm (f), 500 nm (g).