Table 1.
Flow cytometry data showing effects of OEC-CM or SC-CM on Cx expression in ASCs after 24 h and 7 days of culture.
| 24 h |
7 days |
||||||
|---|---|---|---|---|---|---|---|
| Controls | OEC-CM | SC-CM | Controls | OEC-CM | SC-CM | ||
| Cx32 | % positive cells | 8.12 ± 0.5 | 11.29 ± 2.1 | 15.46 ± 3.5 | 10.54 ± 2.3 | 33.10 ± 2.0 | 69.46 ± 2.2 |
| MFI | 1.2 ± 0.4 | 1.4 ± 0.3 | 1.6 ± 0.5 | 1.4 ± 0.3 | 2.3 ± 0.2 | 2.8 ± 0.4 | |
| Cx36 | % positive cells | 8.26 ± 4.1 | 14.36 ± 3.3 | 47.2 ± 3.5 | 0.92 ± 0.2 | 30.02 ± 3.1 | 49.47 ± 3.3 |
| MFI | 1.8 ± 0.3 | 1.9 ± 0.2 | 2.9 ± 0.3 | 1.5 ± 0.2 | 1.9 ± 0.4 | 2.3 ± 0.2 | |
| Cx43 | % positive cells | 66.10 ± 2.5 | 83.90 ± 3.2 | 91.29 ± 2.3 | 75.46 ± 3.2 | 95.31 ± 3.1 | 96.14 ± 2.3 |
| MFI | 2.6 ± 0.5 | 3.3 ± 0.3 | 4.6 ± 0.4 | 2.6 ± 0.6 | 5.0 ± 0.4 | 5.4 ± 0.2 | |
| Cx47 | % positive cells | 0.77 ± 0.2 | 1.39 ± 0.3 | 1.31 ± 0.2 | 0.59 ± 0.1 | 1.33 ± 0.4 | 6.31 ± 0.3 |
| MFI | 1.1 ± 0.2 | 1.2 ± 0.3 | 1.3 ± 0.2 | 1.2 ± 0.1 | 1.7 ± 0.4 | 1.6 ± 0.4 | |
All data are expressed as means and standard deviations (Means ± SDs) of three values. Adipose-derived stem cells (ASCs) cultured in basal medium (controls), in conditioned medium from olfactory ensheathing cells (OEC-CM) or conditioned medium from Schwann cells (SC-CM). Cx, connexin; MFI, mean fluorescence intensity.