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. 2018 Nov 12;46(14):3521–3531. doi: 10.1177/0363546518804807

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© 2018 The Author(s)

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 License (http://www.creativecommons.org/licenses/by-nc-nd/4.0/) which permits non-commercial use, reproduction and distribution of the work as published without adaptation or alteration, without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Enumeration of bone marrow obtained by aspiration and after rasping. Two methods of bone marrow collection: (A) via aspiration with Jamshidi needle and (B) after rasping. (C) Line graphs show population-doubling time of paired aspirated and rasped MSCs between passage 1 and passage 2. n = 6 donors. *P = .006. (D) Paired-sample line graphs showing number of CFU-Fs derived from 1 million mononuclear cells. n = 6 donors. **P < .005. (E) Morphology of MSCs in passage 2, derived from BM obtained by aspiration and after rasping. Scale = 20 μm. (F) Representative flow cytometric histograms showing immunophenotype of passage 2 aspirated and rasped MSCs. asp, aspiration; CFU-F, colony-forming unit–fibroblast; MSC, mesenchymal stem/stromal cell; rasp, rasping.