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. 2018 Nov 12;46(14):3521–3531. doi: 10.1177/0363546518804807

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© 2018 The Author(s)

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 License (http://www.creativecommons.org/licenses/by-nc-nd/4.0/) which permits non-commercial use, reproduction and distribution of the work as published without adaptation or alteration, without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Flow cytometric analysis of CD45, CD271, and CD56 expression in freshly isolated mononuclear cells from paired bone marrow obtained by aspiration and after rasping. (A) After dead cell exclusion with DAPI, CD45– cells were selected, and the expression of CD56 on CD271+ cells was analyzed. Note that CD56 is expressed on rare CD271+ cells and is enriched in rasped samples as compared with the aspirate (n = 6 donors). (B) Percentage of CD271+CD56+ and CD271+CD56– mesenchymal stem/stromal cell subsets in the freshly isolated paired aspirate and rasped samples shown in panel A. Relationship between donor-matched chondrogenic histologic scores and total number of (C) CD271+CD56+ cells and (D) CD271+CD56– cells freshly seeded from corresponding aspirate and rasped samples, by Spearman rank correlation (n = 6 donors). N.S., not significant.