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. Author manuscript; available in PMC: 2019 Nov 15.
Published in final edited form as: Cytoskeleton (Hoboken). 2018 Nov 15;75(10):427–436. doi: 10.1002/cm.21489

Figure 4. Fluorescence splicing reporter analysis for lev-11 E5s.

Figure 4.

(A-C) Schematic illustration of reporter minigenes for lev-11 E5a (E5a-ECFP) (A), E5b (E5b-Venus) (B), and E5c (E5c-HcRed) (C). These reporters were expressed under the control of the ubiquitous eef-1A.1 promoter. Structures of mRNA isoforms that encode the fluorescent proteins are indicated below each minigene. The cDNA cassettes and the predicted ORFs for ECFP, Venus and HcRed are colored in cyan, green and magenta, respectively. Green circles and red diamonds indicate artificially-introduced initiation and termination codons, respectively. Red crosses indicate disruption of the 5’-splice sites for E4b. (D-G) Fluorescence images showing expression of E5a-ECFP (D), E5b-Venus (E) and E5c-HcRed (F). A merged image with ECFP in blue, Venus in green, and HcRed in red, is shown in G. Bar, 100 μm.