Fig. 2.

Modulation in cell cycle and proliferation pattern of neuro-2a under varied serum conditions with presence/absence of retinoic acid. Neuro-2a cells were subjected to culture conditions, namely DMEM + 10% FBS (GpI), DMEM + 10% FBS + 20 μM Retinoic acid (GpII), DMEM + O.1% FBS (GpIII), DMEM + 0.1% FBS + 20 μM Retinoic acid (GpIV) for 24 h, processed as per respective protocols and then scanned using FACS Calibur™. Cell cycle data are represented as the histogram (A–D) and CFSE assay data is represented in bar graphs (E, F) showing mean fluorescence intensity values (MFI values) and cell counts (cells/9.6 cm²) respectively. Values are mean ± S.E. P ≤ 0.05 (α, β, γ), P ≤ 0.01 (αα, ββ, γγ), P ≤ 0.001 (ααα, βββ, γγγ) were considered to be statistically significant. α, Compared to GpI; β, Compared to GpII; γ, Compared to GpIII. Additionally, mean data values, S.E., ANOVA statistics and P. Values from Tukey׳s multiple comparisons (post hoc) are represented besides respective graphical representations.