Fig. 3.

Protein and mRNA expression data for the neuronal marker of differentiation using various serum concentrations in the presence/absence of retinoic acid. A; Represents western blot of ChAT, Rbfox3, GAPDH. B, C; Relative quantification of ChAT and Rbfox3 w.r.t. GAPDH as the reference control. D, E; Real-time PCR data for ChAT and Rbfox3, analyzed by the 2^-ddCt method using SDHA as the reference gene. Values are mean ± S.E. P ≤ 0.05 (α, β, γ), P ≤ 0.01 (αα, ββ, γγ), P ≤ 0.001 (ααα, βββ, γγγ) were considered to be statistically significant. α, Compared to GpI; β, Compared to GpII; γ, Compared to GpIII. Additionally, mean values, S.E., ANOVA, and Tukey׳s multiple comparisons statistics are shown in tabular form below their respective graphs.