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. Author manuscript; available in PMC: 2018 Dec 6.
Published in final edited form as: Reprod Toxicol. 2016 Dec 19;70:70–81. doi: 10.1016/j.reprotox.2016.12.004

Figure 4: Hyaloid vessel assay.

Figure 4:

Figure 4:

Figure 4:

Tg(fli1:EGFP) zebrafish embryos were exposed to 37 test chemicals from 48–120 hpf. GFP positive hyaloid primary branches covering the back of the lens were imaged and quantified on an Olympus SZX16 fluorescence microscope (n = 3 with 5 larvae per well per concentration) (Supplemental Figure S4). (A) Representative negative and positive control (Su) images are shown. Primary hyaloid vessel (HV) branches (white arrows) emerge from the optic disk (asterisk). (B) Representative images and (C) HV vessel quantification for haloperidol, 1-hydroxypyrene, and bisphenol A are shown.