TABLE 2.
Plasmids used in this study
| Plasmid | Relevant genotype | Reference or construction |
|---|---|---|
| pTrc99a | lacIq Ptrc, MCS from pUC18, rrnB(Ter) bla ori pMB1 | 59 |
| pSixA (pJS17) | pTrc99a sixA | NcoI-sixA-F + HindIII-sixA-R |
| pSixA(H8A) (pJS21) | pTrc99a sixA(H8A) | sixA-dead-F + sixA-deadCRIM-R2 |
| pMG91 (pSMART) | pSMART VC BamHI oriV ori2 repE parABC cat | Lucigen Corporation |
| pYcgO (pJS15) | pSMART ycgO | EcoRI-PcvrA-F + HindIII-cvrA-R |
| pBR322 | rop, tet, bla, ori pMB1 | 60 |
| pEB52 | pTrc99a with NcoI restriction site removed | 61 |
| pEIIANtr (pJS37) | pEB52 ptsN-3×FLAG | EcoRI-ptsN-F + HindIII-ptsN-3×FLAG-R2 |
| pRL27 | oriR6Kγ aph oriT PtetA-tnp | 54 |
| pCP20 | λcI857(ts) λpR-FLP repA101(ts) oriR101 bla cat | 50 |
| pEL8 | pCP20 Δcat | 62 |
| pKD3 | oriR6Kγ bla FRT-cat-FRT | 63 |