FIG 3.

Absence of cholesteryl glucosides increased H. pylori cell wall permeability. (A and B) Flow cytometry analysis based on influx rates of EtBr as depicted in the form of PE-A histograms delineating the median fluorescence intensity (MFI) for Hp26695 strain grown in the presence and absence of cholesterol (A). (B) Influx rates of EtBr for Hp26695 and Hp26695Δ421 strains grown for 48 h. (C to F) The bars represent MFIs for wild-type, knockout, and reconstituted strains (as annotated) when treated/incubated with EtBr for 5, 10, and 30 min using cultures grown either at 48 h or 72 h (*, P < 0.05; **, P ≤ 0.01; ***, P ≤ 0.001).