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. 2018 Dec 4;9(6):e01290-18. doi: 10.1128/mBio.01290-18

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FIG 2 — Treatment with drugs inhibiting ergosterol biosynthesis affects flow cytometry patterns. Log-phase-grown H99 cells were treated with indicated drugs for various times. PI-stained cells were analyzed by flow cytometry. (A) Top row shows the histograms of fluorescence intensity (FL3-A) of cells treated with indicated drugs. Middle row shows the forward (x axis) and side (y axis) scatter patterns. Bottom row shows the micrographs of PI-stained cells. (B) Histogram of fluorescence intensity (left panel) and forward-side scatter patterns (right panel) of cells treated with amphotericin B for various times. (C) The effects of azole drugs are reversible. Log-phase YPD-grown cells (0) were treated with indicated drugs for 4 h (+drug). Cells were washed and transferred to YPD without drug for 2, 4, and 6 h (−2h, −4h, −6h). The PI-stained cells were analyzed by flow cytometry. Top row shows the histograms of fluorescence intensity. Bottom row shows the forward-side scatter patterns. Flc, 32 µg/ml of fluconazole; Vor, 4 µg/ml voriconazole; Ter, 1 µg/ml terbinafine; Fen, 3 µg/ml fenpropimorph.