FIG 7.

Partitioning of core biosynthetic proteins of the scytonemin operon between cytoplasm and periplasm by proteomic analyses of osmotic shock lysates. Target protein relative abundance ratios to three different known periplasm-targeted proteins are plotted against the strength of lysis buffer used. Proteins localized to the cytoplasm should show an increase in ratio with buffer strength, whereas ratios of proteins partitioning preferentially to the periplasm should remain invariant or decrease. All ratios were normalized to 1 at 0.3 M sucrose for ease of graphing, and P values are included for each target protein data set (α = 0.01).