FIG 3.

(A) Shown is the region of the mtrR-mtrC that was PCR amplified from chromosomal DNA of strain CDC2 (blue line) used to transform strain FA19. The region of recombination in transformant strain CR.100 is shown by a blue line. The nucleotide sequences of the mtrR/mtrC promoter region (mtrCDE coding strand) from strains FA19, CDC2, CR.100, and CR.101 are shown below. The translation start codon for mtrR is shown in green. The −10 and −35 hexamers of the mtrR and mtrCDE promoters are boxed. The TSS sites for both promoters are shown by asterisks. The red arrow shows the point mutation in the −35 hexamer of the mtrCDE sigma-70 promoter. Differences in nucleotide sequence or deletions are highlighted in red. (B) The predicted amino acid sequences of MtrR produced by strains FA19, CDC2, CR.100, CR.101, and CR.102 are shown. Differences at sites 79, 183, and 197 are highlighted in red and with asterisks.