Figure 4.

TrkB receptor agonist rescues synaptic impairment when applied seven days after CA/CPR. (a) Experimental design for LTP experiments. (b) Time course of fEPSP slope from mice seven days after CA/CPR (black, n = 6) and paired slices bath applied with 7,8 dihydroxyflavone (7,8 DHF, 250 nM, n = 6, red). Arrow indicates timing of theta burst stimulation (TBS, 40 pulses, 100 Hz). (c) Quantification of change in fEPSP slope after 60 min following TBS normalized to baseline. Sham and seven-day CA/CPR slices were paired with and without 7,8 DHF and paired experiments are indicated by the line between the pairs. (d) Experimental design for contextual fear conditioning in relation to 7,8 DHF injection. (e) Quantification of freezing behavior 24 h after contextual fear conditioning in a novel environment in sham or cardiac arrest mice administered vehicle or 7,8 DHF (5 mg/kg). Each dot represents one animal used for behavior. *p < 0.05 by paired t-test for electrophysiology and ANOVA for behavior.

LTP: long-term potentiation; CA/CPR: cardiac arrest/cardiopulmonary resuscitation.