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. 2018 Oct 22;38(12):2209–2222. doi: 10.1177/0271678X18803623

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Figure 1. — Two pools of PgP/caveolar proteins occur in membranes from rat brain microvessel isolates. (a) Representative immunoblot of total PgP and α-tubulin in microvessel isolates from saline-injected control (S) and ʎ carrageenan-injected (C) animals. (n = 4 pools of three rats). (b) Quantitation of PgP corrected for α-tubulin as a loading control in microvessel isolates from saline-injected control (SAL) and ʎ carrageenan-injected (CAR) animals. Values are the mean + SEM (n = 4 pools of three rats). (c) Total protein (closed circles), cholesterol (open circles) and density (closed diamonds) profile of the OptiPrep gradient fractions loaded with a sample of rat brain microvessel isolate. Values are the mean +/− SEM (n = 3 pools of three rats). (d) Representative immunoblots indicating the gradient fractions that contain specific proteins from samples from saline-injected control (SAL) and ʎ carrageenan-injected (CAR) animals (n = 3 pools of three rats). PgP: P-glycoprotein; CAV1: caveolin1; PTRF/cavin1: polymerase 1 and transcript release factor; PRKCDBP/cavin3: protein kinase C delta binding protein; SDPR/cavin2: serum deprivation response protein; GLUT1:glucose transporter1.