Figure 1.
The four-core-genotype (FCG) mouse model to determine the role of sex chromosomes in ischemic sensitivity and outcome. (a) Schematic of the generation of FCG mice in which the Sry gene (the testis-determining gene; sex-determining region, Y chromosome) is removed from the Y chromosome and inserted on an autosome (Chromosome 3).139 This produces XX and XY mice with testes, and XX and XY mice with ovaries, allowing for the independent assessment of the effects of sex chromosome complement. (b) PCR showing the presence of the Sry in XYM and XXM (gonadal males) and the absence of Sry in XXF and XYF (gonadal females). Ymt identifies the Y chromosome and Myo is used as a positive control.