Fig. 2.

Effects of BPRBE on iNOS gene expression in S. Typhimurium-infected macrophages. a iNOS mRNA expression profiles assessed by RT-PCR. RAW 264.7 cells were cultivated with or without BPRBE for 24 h. The cells were then infected with S. Typhimurium SL1344 for 8 h. After infection, total RNA was purified and an iNOS mRNA expression profile was determined using RT-PCR analysis. b Assessment of iNOS protein expression profiles by Western blot analysis. RAW 264.7 cells treated with BPRBE for 24 h and then infected with S. Typhimurium were lysed and the iNOS protein in the cell lysate was then identified by Western blotting using rabbit anti-mouse iNOS polyclonal antibody. The relative proportions of iNOS mRNA and polypeptide are expressed as the R. E. (relative expression) values calculated from iNOS/β-actin gene expressions. Figures represent results from at least three individual experiments