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. 2018 Dec 5;11:625. doi: 10.1186/s13071-018-3198-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Anti-rTs31 antibody responses and immune protection in vaccinated mice. a Total anti-rTs31 IgG in serum of mice vaccinated with rTs31 (MBP tag, adjuvant or PBS) at various times post-vaccination. b Specific IgG subtypes (IgG1 and IgG2a) responses to rTs31 at various times post-vaccination. The OD value from each group is the mean ± SD of the antibody levels (n = 10). The vaccination time is indicated by solid triangle, and the challenge time is indicated by a solid arrow. c and d Adult worm (c) and muscle larvae (d) burden of vaccinated mice after larval challenge. The data are presented as the mean ± SD of each group (n = 10). Asterisks indicate a statistical difference (*P < 0.001) in worm burden in rTs31-immunized group relative to the control groups (MBP, adjuvant and PBS group)