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. 2018 Dec 5;15:336. doi: 10.1186/s12974-018-1367-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — 21 days after LPS injection, rat brain tissue glycoproteins show reduced staining for MAL-II lectin and increased staining for PNA lectin consistent with their desialylation. a, b Representative glycoprotein patterns of brain tissue homogenates of Sham (lanes 2–3) and LPS-injected (lanes 4–5) rat pups at P24 after injection. Following SDS-PAGE, blots were stained with biotinylated peanut agglutinin (PNA) lectin, specific for Gal-GalNAc residues and biotinylated Maackia amurensis II (MAL II) lectin, specific for α2–3-linked sialic acids. The bands showing apparent difference in the intensity between LPS and Sham samples are boxed. c, d Intensity of lectin-positive bands normalized to the level of total protein. For statistical analysis, five animals from each group were used; *p < 0.05, **p < 0.01