Table 1.
List of primers used in quantitative RT-PCR analysis, antibodies and lectins used in the experiments and their working dilutions
| Gene | Forward (5′ to 3′) | Reverse (5′ to 3′) | Reference |
| Gapdh | AAGGTCGGTGTGAACGGATT | TGAACTTGCCGTGGGTAGAG | [63] |
| Neu1 | GTAGCACGTGGTCCTCTACG | ATCGTGATCGTGTTTGGGCT | Primer Blast |
| Neu3 | CTCAGTCAGAGATGAGGATGCT | GTGAGACATAGTAGGCATAGGC | [64] |
| Neu4 | CCGGACTGTGGTTGGTAGAC | ACCAGGGAGCGTAAAGCAAT | Primer Blast |
| Antibody/lectin | Working dilution | Catalog number, company | |
| Anti GFAP (GA5), mouse mAb | 1:300 | 3670, Cell Signaling Technology | |
| Anti-PSA, mouse mAb | 1:600 | [65] | |
| Anti-NeuN, mouse mAb | 1:200 | MAB377, EMD Millipore Corporation | |
| Anti-NeuN, guinea pig polyclonal | 1:300 | ABN90P. EMD Millipore Corporation | |
| Anti-LAMP1 antibody—lysosome marker | 1:1500 | ab24170, Abcam | |
| Goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488 | 1:1500 | A-11034, Thermo Fisher Scientific | |
| Goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 555 | 1:1500 | A-21422, Thermo Fisher Scientific | |
| Goat anti-mouse IgG (H+L), cross-adsorbed secondary antibody, Alexa Fluor 488 | 1:500 | A11001, Invitrogen | |
| Donkey anti-guinea pig IgG antibody, Cy3 conjugate 555 | 1:750 | AP193C, EMD Millipore Corporation | |
| Isolectin GS-IB4 from Griffonia simplicifolia, Alexa Fluor® 568 conjugate | 1:200 | I21412, Thermo Fisher Scientific | |
| Biotinylated peanut agglutinin lectin | 1:10,000 | B-1075, Vector Laboratories | |
| Biotinylated Maackia amurensis lectin II | 1:20,000 | B-1265, Vector Laboratories | |
| Streptavidin-horseradish peroxidase conjugate | 1:1000 | RPN1231-100UL, GE Healthcare Life Sciences | |